Soluble chemokine receptor CXCR4 is present in human sera

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2011 English Publisher:Elsevier BVJournal:Analytical Biochemistry, volume 414, pages 202-207 (issn: 0003-2697,

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Authors: Etienne, Malvoisin; Jean-Michel, Livrozet; Djamila, Makloufi; Nadine, Vincent;

doi: 10.1016/j.ab.2011.03.022

pmid: 21440527

Soluble chemokine receptor CXCR4 is present in human sera

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Abstract

A soluble form of the chemokine receptor CXCR4 was detected in human sera by isoelectric focusing and Western blotting. Sera of patients and normal subjects were analyzed using a panel of specific antibodies. Compared with controls, high levels of serum CXCR4 were found in patients with inflammatory bowel diseases. Serum CXCR4 levels in the majority of HIV patients were similar to those in healthy controls. A sensitive polyclonal antibody was developed in rabbit immunized with a maltose binding protein (MBP) construct expressing the full-length CXCR4. Using anti-MBPCXCR4 antibody, the level of CXCR4 in sera of a majority of patients with fibrosis was very low. The potential of serum CXCR4 as a new diagnostic biomarker warrants further investigation.

Related Organizations

Hôpital Édouard-Herriot
France
Centre Hospitalier Universitaire de Bordeaux
France
Hospices Civils de Lyon
France

Keywords

Receptors, CXCR4, Recombinant Fusion Proteins, Blotting, Western, HIV Infections, Antibodies, Maltose-Binding Proteins, Animals, Humans, Rabbits, Isoelectric Focusing, Biomarkers

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