Metal particle-enhanced fluorescent immunoassays on metal mirrors
Copyright policy )Metal particle-enhanced fluorescent immunoassays on metal mirrors
We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.
- University of Calgary Canada
- University of Maryland, Baltimore United States
- Macquarie University Australia
- University of North Texas United States
Silver, Myoglobin, Fluoroimmunoassay, Metal Nanoparticles, Reproducibility of Results, Models, Theoretical, Microscopy, Atomic Force, Metals, Immunoglobulin G, Animals, Nanotechnology, Rabbits
Silver, Myoglobin, Fluoroimmunoassay, Metal Nanoparticles, Reproducibility of Results, Models, Theoretical, Microscopy, Atomic Force, Metals, Immunoglobulin G, Animals, Nanotechnology, Rabbits
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