Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites

descriptionPublicationkeyboard_double_arrow_right Article 01 May 2006 English Publisher:Elsevier BVJournal:Analytical Biochemistry, volume 352, pages 129-134 (issn: 0003-2697,

Copyright policy )

Authors: Shouzou, Wei; James J, Brittin; John R, Falck; Siddam, Anjaiah; Kasem, Nithipatikom; Lijie, Cui; William B, Campbell; +1 Authors

doi: 10.1016/j.ab.2006.01.005

pmid: 16480681

Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites

- Summary
- Subjects
- Metrics

Abstract

An HPLC method for the chiral analysis of the four regioisomeric epoxyeicosatrienoic acids (EETs) is described. The cytochrome P450 arachidonic acid epoxygenase metabolites are resolved, without the need for derivatization, by chiral-phase HPLC on a Chiralcel OJ column. Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase.

Related Organizations

Vanderbilt University
United States
Medical College of Wisconsin
United States
The University of Texas Southwestern Medical Center
United States

Keywords

Male, Vasodilator Agents, Stereoisomerism, Cytochrome P-450 CYP2J2, Rats, Rats, Sprague-Dawley, 8,11,14-Eicosatrienoic Acid, Cytochrome P-450 Enzyme System, Liver, Microsomes, Liver, Oxygenases, Animals, Chromatography, High Pressure Liquid

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	18
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%