Characterization of GPCRs in extracellular vesicle (EV)
Characterization of GPCRs in extracellular vesicle (EV)
Extracellular vesicle (EV) are tiny membranous vesicles usually <500nm in size that recently emerged as a new paradigm in human intercellular signaling. EVs have shown a promising role in development of diagnostic markers in many pathophysiological disorders. The presence of chemosensory and therapeutically relevant G protein-coupled receptors (GPCRs) on EV membranes is poorly characterized. Here, we compare different methods including ultracentrifugation and polymer-charge-based separation to isolate EVs from cell culture media and human saliva. The presence of bitter taste GPCRs (T2R4 and T2R38) and a class A GPCR angiotensin II type 1 receptor on these EVs was characterized by qPCR, ELISA, and immunotransmission electron microscopy.
- Apollo Hospitals India
- Apollo Hospitals India
- University of Manitoba Canada
Taste Receptors, Type 2, Enzyme-Linked Immunosorbent Assay, Real-Time Polymerase Chain Reaction, Receptor, Angiotensin, Type 1, Cell Line, Receptors, G-Protein-Coupled, Extracellular Vesicles, Microscopy, Electron, Transmission, Humans, Microscopy, Immunoelectron, Oligopeptides, Ultracentrifugation
Taste Receptors, Type 2, Enzyme-Linked Immunosorbent Assay, Real-Time Polymerase Chain Reaction, Receptor, Angiotensin, Type 1, Cell Line, Receptors, G-Protein-Coupled, Extracellular Vesicles, Microscopy, Electron, Transmission, Humans, Microscopy, Immunoelectron, Oligopeptides, Ultracentrifugation
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