The application of electrophysiological techniques to tissue culture is still evolving. We have attempted in this chapter to give a practical summary of intracellular recording techniques used in our laboratory, as well as give some examples of new experimental strategies and electrophysiological methods that should provide further information on a number of interesting neurobiological questions. The combination of an increasing knowledge of the cell biology of cultured neurons and advances in electrophysiology should continue to be a fruitful interaction.