
pmid: 15539235
Determination enzymatique et spectrophotometrique de l'adenosine diphosphate (ADP) et de l'adenosine monophosphate (AMP). Phosphorylation de l'ADP par une pyruvate kinase puis reduction du pyruvate en presence de NADH. Mesure de la consommation de NADH au spectrophotometre a 340 nm. Meme reaction pour l'AMP en ajoutant une myokinase
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