Organization and sequence of the SalI restriction-modification system
Copyright policy )Organization and sequence of the SalI restriction-modification system
The organization and nucleotide (nt) sequences were determined for the genes encoding the SalI restriction and modification (R-M) system (recognition sequence 5'-GTCGAC-3') from Streptomyces albus G. The system comprises two genes, salIR, coding for the restriction endonuclease (ENase, R.SalI; probably 315 amino acids (aa), a predicted M(r) of 35,305; product, G'TCGAC) and salIM, coding for the methyltransferase (MTase, M.SalI; probably 587 aa, a predicted M(r) of 64,943; product, GTCGm6AC). The genes are adjacent, they have the same orientation, and they occur in the order salIR then salIM. R.SalI contains a putative magnesium-binding motif similar to those at the active sites of R.EcoRI and R.EcoRV, but otherwise it bears little aa sequence similarity to other ENases. M.SalI is a member of the m6A gamma class of MTases. In aa sequence it resembles M.AccI, another m6A gamma-MTase whose recognition sequence includes the SalI recognition sequence as a subset.
- University of Oviedo Spain
- New England Biolabs United States
Base Composition, Site-Specific DNA-Methyltransferase (Adenine-Specific), Base Sequence, Sequence Homology, Amino Acid, Transcription, Genetic, Molecular Sequence Data, Restriction Mapping, Chromosome Mapping, Regulatory Sequences, Nucleic Acid, Streptomyces, Genes, Bacterial, Genes, Regulator, Amino Acid Sequence, Codon, Deoxyribonucleases, Type II Site-Specific
Base Composition, Site-Specific DNA-Methyltransferase (Adenine-Specific), Base Sequence, Sequence Homology, Amino Acid, Transcription, Genetic, Molecular Sequence Data, Restriction Mapping, Chromosome Mapping, Regulatory Sequences, Nucleic Acid, Streptomyces, Genes, Bacterial, Genes, Regulator, Amino Acid Sequence, Codon, Deoxyribonucleases, Type II Site-Specific
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