XcmI as a universal restriction enzyme for single-stranded DNA
Copyright policy )XcmI as a universal restriction enzyme for single-stranded DNA
Single-stranded DNA can be cleaved into defined fragments at any predetermined site by interaction with a specially designed oligodeoxyribonucleotide (oligo) adaptor and the class-IIN restriction endonuclease, XcmI. The oligo adaptor has the structure [sequence: see text]. Upon hybridization to the target DNA through the central 9-nucleotide region and with the addition of XcmI, the template DNA is specifically cleaved to near completion. Hairpin structures on the template close to the hybridization site reduce the efficacy of cleavage.
- Chinese University of Hong Kong China (People's Republic of)
Base Sequence, Oligodeoxyribonucleotides, Molecular Sequence Data, DNA, Single-Stranded, Nucleic Acid Conformation, Deoxyribonucleases, Type II Site-Specific, Substrate Specificity
Base Sequence, Oligodeoxyribonucleotides, Molecular Sequence Data, DNA, Single-Stranded, Nucleic Acid Conformation, Deoxyribonucleases, Type II Site-Specific, Substrate Specificity
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