Mapping the order of DNA restriction fragments
Copyright policy )Mapping the order of DNA restriction fragments
A straightforward method was designed for mapping the order of DNA restriction fragments obtained by a double and two single digestions, without the necessity of using a computer or a radioactive label. All possible solutions compatible with a pre-set level of error in the determination of sequence lengths are obtained. The primary assumptions are given, and the appropriate modifications of the algorithm are presented as a function of any assumptions one is unable (or unwilling) to make. Use of the method in connection with end-labeled fragments is also described.
- University of Wisconsin–Madison United States
- University of Wisconsin System United States
- University of Wisconsin–Oshkosh United States
- Northern Michigan University United States
- Los Alamos National Laboratory United States
Genetic Vectors, Statistics as Topic, Methods, Chromosome Mapping, DNA, DNA Restriction Enzymes, Cloning, Molecular
Genetic Vectors, Statistics as Topic, Methods, Chromosome Mapping, DNA, DNA Restriction Enzymes, Cloning, Molecular
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