Abstract 1. 1. Robinia pseudo-acacia honey and Apis mellifera food gland amylase display optimal activity at pH 5·6–5·9 and 45°C, with a persistent feeble activity at 80°C. The chloride ions (0·1 M) are the activators of both enzymes. 2. 2. R. pseudo-acacia nectar and Sambucus nigra pollen amylase, on the other hand, displayed well-marked pH peaks at 7·2 and 5·9 respectively. The optimum temperature was 45°C, with total inactivity at 75°C. 3. 3. It is therefore considered that honey amylase may be treated as being of animal origin.