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A revaluation of the haemoglobin electrophoretogram of the wistar rat

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1971Publisher:Elsevier BVJournal:Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, volume 40, pages 155-163 (issn: 0305-0491,

Authors: Norman Maclean; Andrew R.E. Shaw;

doi: 10.1016/0305-0491(71)90072-1

pmid: 5141393

A revaluation of the haemoglobin electrophoretogram of the wistar rat

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Abstract

Abstract 1. 1. Haemoglobins of Wistar rats have been examined by starch and polyacrylamide gel electrophoresis 2. 2. Two haemoglobins are produced during yolk sac erythropoiesis, and two additional haemoglobins appear during liver erythropoiesis. 3. 3. All four haemoglobins persist in the adult. 4. 4. Electrophoretograms of adult haemolysates demonstrate the loss of one haemoglobin, and the appearance of an additional fast-moving band. 5. 5. Evidence is presented that glutathione may bind to that haemoglobin increasing its electrophoretic mobility.

Related Organizations

University of Southampton
United Kingdom

Keywords

Electrophoresis, Time Factors, Alkylation, Iodoacetates, Rats, Inbred Strains, Glutathione, Rats, Dithiothreitol, Embryonic and Fetal Development, Hemoglobins, Liver, Acetamides, Animals, Erythropoiesis, Female, Fetal Hemoglobin, Mercaptoethanol

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Fields of Science (3) View all

medical and health sciences

basic medicine

Fields of Science

medical and health sciences

basic medicine

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