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Localization of a VP3 epitope of infectious bursal disease virus

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1991 English Publisher:Elsevier BVJournal:Virology, volume 184, pages 805-807 (issn: 0042-6822,

Authors: Mittur N. Jagadish; Ahmed A. Azad;

doi: 10.1016/0042-6822(91)90457-m

pmid: 1716030

Localization of a VP3 epitope of infectious bursal disease virus

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Abstract

An immunodominant region of VP3, one of the two structural proteins of infectious bursal disease virus (IBDV strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in Escherichia coli, followed by immunoblot analysis of the synthesized products. The epitope located within 58 amino acids reacted very strongly with a mouse monoclonal antibody (MAb 17/80) raised against IBDV 002-73. This immunodominant region may be useful in serodiagnosis of IBDV infection in poultry.

Related Organizations

Commonwealth Scientific and Industrial Research Organisation
Australia

Keywords

DNA Mutational Analysis, Molecular Sequence Data, Restriction Mapping, Proteins, Infectious bursal disease virus, Epitopes, Capsid, Capsid Proteins, Amino Acid Sequence, Cloning, Molecular, Antigens, Viral

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