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Characterization of human papovavirus RFV: Use of iodinated viral DNA to detect viral DNA sequences in cellular DNA

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1977Publisher:Elsevier BVJournal:Virology, volume 77, pages 856-859 (issn: 0042-6822,

Authors: R.M. Dougherty; R. Miao;

doi: 10.1016/0042-6822(77)90506-2

pmid: 324115

Characterization of human papovavirus RFV: Use of iodinated viral DNA to detect viral DNA sequences in cellular DNA

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Abstract

Abstract The amount of viral DNA present in a viral transformed cell line has been determined using saturation reassociation of cellular DNA and 125 I-labeled viral DNA. The virus, RFV, is a new human papovavirus which was used to transform hamster embryo cells in vitro . Only 40% of the viral genome is present in the transformed cells and less than 5% was present in cells from normal hamster livers. The high specific activity of the [ 125 I]RFV DNA permitted the quantitative determination of RFV sequences present using less than 260 μg of cellular DNA.

Related Organizations

Syracuse University
United States

Keywords

Hot Temperature, Base Sequence, Nucleic Acid Denaturation, Cell Line, Immunoenzyme Techniques, Iodine Radioisotopes, Cell Transformation, Neoplastic, Cricetinae, DNA, Viral, Nucleic Acid Renaturation, Animals, Humans, Polyomaviridae, Papillomaviridae

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