descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1991 English Publisher:Elsevier BVJournal:Journal of Immunological Methods, volume 138, pages 165-171 (issn: 0022-1759,

Authors: Fritz Gamerith; Johannes Menzel; Uriwan Youngchaiyud; Gernot Kolarz; O. Scherak;

doi: 10.1016/0022-1759(91)90164-b

pmid: 1851794

A method to differentiate between anti-C1q antibodies and C1q-binding immune complexes using collagenase-digested solid phase C1q

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Abstract

A method for the detection of circulating immune complexes in the presence of autoantibodies to C1q is described. Solid phase C1q-digestion with bacterial collagenase results in the elimination of the collagen-like region of C1q. Binding of model immune complexes to this modified solid phase C1q is practically unaltered, while reactivity of anti-C1q antibodies is abolished by this procedure. In conjunction with an ELISA using the collagen-like region of C1q as antigen this modified C1q solid phase assay may be used to determine immune complexes and anti-C1q antibodies in the sera of patients with autoimmune rheumatic diseases.

Related Organizations

Mahidol University
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Siriraj Hospital
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Ludwig Boltzmann Institute for Digital Health and Prevention
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Ludwig Boltzmann Gesellschaft
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Keywords

Microbial Collagenase, Complement C1q, Rheumatic Diseases, Antibodies, Monoclonal, Humans, Enzyme-Linked Immunosorbent Assay, Antigen-Antibody Complex, Autoantibodies, Immunophenotyping

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