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Localization of anti-nuclear antibodies on human metaphase chromosomes

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1974Publisher:Elsevier BVJournal:Journal of Immunological Methods, volume 5, pages 21-31 (issn: 0022-1759,

Authors: J.I. Watson; R.B. Church; Marvin J. Fritzler;

doi: 10.1016/0022-1759(74)90042-8

pmid: 4601391

Localization of anti-nuclear antibodies on human metaphase chromosomes

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Abstract

Abstract A technique has been developed for the in vitro demonstration of anti-nuclear antibodies (ANA) in the sera of patients with systemic lupus erythematosus (SLE) utilizing human metaphase chromosomes in situ as substrate. The selective removal of histone, acidic protein, RNA and treatment with 2% formamide improves the binding of antibodies to DNA as demonstrated by fluorescein-conjugated and horseradish peroxidase-conjugated goat anti-human IgG. This technique is not only important because of the relative ease of demonstration of ANA but may be a valuable tool in the investigation of the pathogenesis of SLE.

Related Organizations

University of Calgary
Canada

Keywords

Formamides, Histocytochemistry, Fluorescent Antibody Technique, Mitosis, Nucleic Acid Hybridization, Chromosomes, Peroxidases, Antibodies, Antinuclear, Methods, Humans, Lupus Erythematosus, Systemic

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