Abstract Results are presented on the effect of several variables on a method for the determination of glucose using a polarograph with a Clark-type O 2 electrode and glucose oxidase. Glucose concentration [ G ] was proportional to the rate of O 2 consumption |− Δ O 2 / Δt | in the 5–800 μg/ml range. With fixed [ G ], |; ΔO 2 / Δt | increased proportionately with enzyme concentration [ E ] in the 0–1.0 mg/ml range. Glycerol [ Gy ] added to stabilize enzyme solutions and facilitate analysis of particulate samples depressed the rate of O 2 consumption in a linear fashion. When [ G ], [ E ], [ Gy ] were varied by changing the final volume in the reaction chamber, values for |− Δ O 2 / Δt | declined hyperbolically. Results indicate that the method offers considerable potential for adaptability to a wide range of analytical conditions.