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Synthesis of polypseudouridylic acid by polynucleotide phosphorylase

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1963Publisher:Elsevier BVJournal:Biochimica et Biophysica Acta, volume 72, pages 353-361 (issn: 0006-3002,

Authors: Irving H. Goldberg; Murray Rabinowitz; L. Sasse;

doi: 10.1016/0006-3002(63)90254-3 , 10.1016/0926-6550(63)90352-9

pmid: 13986657

Synthesis of polypseudouridylic acid by polynucleotide phosphorylase

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Abstract

Abstract 1. 1. Polypseudouridylic acid was synthesized in good yield from pseudouridine diphosphate by polynucleotide phosphorylase (EC 2.7.7.8) of Micrococcus lysodeikticus. Primer trinucleotide, pApApA markedly increased the synthetic reaction rate but did not affect the [32P]Pi-pseudouridine diphosphate exchange. 2. 2. The rate of polypseudouridylic acid formation was usually 2 to 3 times slower than the rate of synthesis of polyuridylic acid.

Related Organizations

University of Chicago
United States

Keywords

Polyribonucleotide Nucleotidyltransferase, Transferases, Uracil Nucleotides, Polynucleotides, Nucleotidyltransferases

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