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Radioimmunoassay of nicotine

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1975Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 64, pages 574-580 (issn: 0006-291X,

Authors: Tatsuya Ishiguro; Shigeru Matsukura; Hitoshi Matsuyama; Noboru Sakamoto; Hiroo Imura; Hideo Muranaka; Taeko Tamada;

doi: 10.1016/0006-291x(75)90360-5

pmid: 1170854

Radioimmunoassay of nicotine

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Abstract

Abstract A sensitive and specific radioimmunoassay of nicotine was developed using antisera raised against 6-(p-aminobenzamido) nicotine coupled to bovine serum albumin. Inhibition studies with various nicotine analogues revealed that the antisera are highly specific for both the N-methylpyrrolidine ring and the pyridine ring of nicotine, and especially for the structural changes of the former. The use of these antisera in an assay of nicotine in biological fluids is desirable, since the pyrrolidine ring of nicotine is first metabolized in vivo and antibodies must, therefore, discriminate nicotine from other nicotine metabolites.

Related Organizations

Japan Tobacco (United States)
United States
Kobe University
Japan

Keywords

Nicotine, Pyrrolidines, Pyridines, Immune Sera, Radioimmunoassay, Serum Albumin, Bovine, Benzamides, Animals, Aminobenzoates, Rabbits

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	26
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%