Determination of elastolytic activity with elastin-rhodamine
Copyright policy )Determination of elastolytic activity with elastin-rhodamine
Abstract Elastin covalently labeled with rhodamine-B-isothiocyanate has been found to be a sensitive substrate for analysis of elastolytic activity in comparison to elastin-orcein and elastin-Congo red. Using elastin-rhodamine in a 20-min test, elastase concentrations as low as 0.1 μg/ml are accurately assayed. Variations in analytical parameters such as temperature, pH, and particle size of the substrate greatly influence the rate of elastolysis.
- University of Mary United States
- Washington University in St. Louis United States
Binding Sites, Pancreatic Elastase, Rhodamines, Swine, Elastin, Kinetics, Spectrometry, Fluorescence, Xanthenes, Methods, Animals, Colorimetry, Indicators and Reagents, Protein Binding
Binding Sites, Pancreatic Elastase, Rhodamines, Swine, Elastin, Kinetics, Spectrometry, Fluorescence, Xanthenes, Methods, Animals, Colorimetry, Indicators and Reagents, Protein Binding
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