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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Analytical Biochemis...arrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Analytical Biochemistry
Article . 1975 . Peer-reviewed
License: Elsevier TDM
Data sources: Crossref
Analytical Biochemistry
Article . 1975
Data sources: Europe PubMed Central
Analytical Biochemistry
Article
Data sources: Microsoft Academic Graph
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A modified assay of 3′:5′-cyclic-AMP phosphodiesterase

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1975Publisher:Elsevier BVJournal:Analytical Biochemistry, volume 63, pages 388-399 (issn: 0003-2697, Copyright policy )
Authors: R. J. Boudreau; G.I. Drummond;

doi: 10.1016/0003-2697(75)90361-9

pmid: 164789

A modified assay of 3′:5′-cyclic-AMP phosphodiesterase

Abstract

Abstract A modification of the assay of cyclic nucleotide phosphodiesterase involving batch use of Dowex 1 anion exchange resin is described which allows for quantitative recovery of adenosine, guanosine, and their metabolites from the resin slurry. The assay described is suitable for use in crude preparations containing purine catabolizing enzymes. A standardized procedure for determining kinetic parameters of cyclic AMP hydrolysis is also discussed. This procedure was used in the partial characterization of the kinetics of cyclic AMP hydrolysis by rat and rabbit heart supernatant fractions.

Related Organizations
Keywords

Time Factors, Phosphoric Diester Hydrolases, Myocardium, Chromatography, Ion Exchange, Rats, Kinetics, Cytosol, Cyclic AMP, Methods, Animals, Rabbits

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    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 163
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
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    		 Top 1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
163
Average
Top 1%
Top 1%
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