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The ionization constant of nicotinamide-adenine dinucleotide

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1969Publisher:Elsevier BVJournal:Analytical Biochemistry, volume 29, pages 149-153 (issn: 0003-2697,

Authors: A.L. Underwood; C.E. Moore;

doi: 10.1016/0003-2697(69)90018-9

pmid: 4305639

The ionization constant of nicotinamide-adenine dinucleotide

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Abstract

The pKa of carefully purified oxidized nicotinamide adenine dinucleotide has been determined by potentiometric titration with sodium hydroxide at 25°. Values are reported for six ionic strengths up to 0.250. Extrapolation to zero ionic strength yields 3.88 ± 0.02 for the thermodynamic pKa.

Related Organizations

Emory University
United States

Keywords

Osmolar Concentration, Potentiometry, Thermodynamics, Hydrogen-Ion Concentration, NAD

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