Expression of small nucleolar RNAs in leukemic cells
Copyright policy )Expression of small nucleolar RNAs in leukemic cells
Small nucleolar RNAs (snoRNAs) direct sequence-specific modifications to ribosomal RNA. We hypothesized that the expression of snoRNAs may be altered in leukemic cells.The expression of snoRNAs was analyzed in various leukemic cell lines by massive parallel sequencing (SOLiD). Quantitative real-time PCR (RT-qPCR) was used to validate the expression profiles.Our results show characteristic differences in the expression patterns of snoRNAs between cell lines representing the main subgroups of leukemia, AML, pre-B-ALL and T-ALL, respectively. In RT-qPCR analyses, several snoRNAs were found to be differentially expressed in T-ALL as compared to pre-B-ALL cell lines.snoRNAs are differentially expressed in various leukemic cell lines and could, therefore, be potentially useful in the classification of leukemia subgroups.
- Tampere University Hospital Finland
- Tampere University Finland
- Åbo Akademi University Finland
- University of Turku Finland
Leukemia, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, ta1182, HL-60 Cells, Sequence Analysis, DNA, ta3111, Gene Expression Regulation, Neoplastic, Jurkat Cells, Cell Line, Tumor, Cluster Analysis, Humans, RNA, Small Nucleolar, RNA, Neoplasm, ta119, Gene Library, HeLa Cells, Oligonucleotide Array Sequence Analysis
Leukemia, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, ta1182, HL-60 Cells, Sequence Analysis, DNA, ta3111, Gene Expression Regulation, Neoplastic, Jurkat Cells, Cell Line, Tumor, Cluster Analysis, Humans, RNA, Small Nucleolar, RNA, Neoplasm, ta119, Gene Library, HeLa Cells, Oligonucleotide Array Sequence Analysis
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