In the present study, two bacteria Pseudomonas sp. and Bacillus sp. resistant to the crude oil were isolated from the Khor Musa sediments, Persian Gulf, Iran. Hemolysis, oil spreading and drop collapse methods were applied to measure the ability of bacteria in producing the biosurfactant. Eventually, the bacterial emulsification activity was reviewed in the presence of crude oil and kerosene. Pseudomonas sp. showed a clear zone around a colony on blood agar medium and made a 2.7-cm-diameter halo. The results of the emulsification test for crude oil and kerosene indicated that Pseudomonas sp. emulsified approximately a half of the kerosene within 2 h, whereas the rate of the kerosene emulsification was lower in Bacillus sp. as emulsification percentage reached to 41.2 % within 48 h. Moreover, the crude oil emulsion was only observed in Pseudomonas sp. The results of this article suggested using the ability of Pseudomonas sp. in producing surfactant to accelerate the hydrocarbons bioremediation.