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Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

descriptionPublicationkeyboard_double_arrow_right Article 06 Sep 2011 India Publisher:Springer Science and Business Media LLCJournal:Indian Journal of Virology, volume 22, pages 142-145 (issn: 0970-2822, eissn: 0974-0120,

Authors: KADAM, S; SHERIKAR, A; PINGALE, V;

doi: 10.1007/s13337-011-0052-1

pmid: 23637517

pmc: PMC3550737

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

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Abstract

Present study was undertaken to compare various routine laboratory diagnostic tests for rabies detection. Seller's staining, mouse inoculation test (MIT), Dot-ELISA, Agar gel precipitation test (AGPT) and counter immunoelectrophoresis test (CIET) were the main basic tests performed in the laboratory for the rabies diagnosis. Out of 200 brain specimens, Negri bodies were observed in 52 brain samples by Seller's staining. Rabies virus was isolated in 56 samples by intra-cerebral inoculation in newborn Swiss-albino mice. Dot-ELISA and AGPT could detect rabies antigen in 55 and 57 samples respectively. Comparative analysis revealed that the CIET is the most sensitive and rapid test among performed diagnostic tests.

Country

India

Related Organizations

Indian Institue of Technology, Bombay
India
Norwegian Institute for Cultural Heritage Research
Norway
Veterinary College, Mathura
India
Indian Institute of Technology Bombay
India
Bombay Veterinary College
India

Keywords

Mouse Inoculation Test, Enzyme-Immunoassay, Rabies, India, Mit, Ciet, Fluorescent-Antibody Test, Virus, Mice, Antigen, Incubation Period, Animals, Modified Counterimmunoelectrophoresis Test, Seller'S Stain, Dot-Elisa

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