Mortars are heterogeneous building materials whose raw materials, manufacturing processes and application conditions have evolved continuously throughout history. The fact that apparently small changes in the components or dosages of mortars can affect their overall performance in the masonry makes the study of historic mortars a complex task that needs to be tackled via a multidisciplinary approach, with the support of complementary analytical techniques from the field of chemistry, mineralogy, physics and engineering, among others. This review is intended to be a useful tool for researchers working in the field of archaeology and/or cultural heritage conservation, as it offers a complete overview of the most widely accepted analytical techniques and physical-mechanical tests used in the characterisation of historic mortars and plasters. Although the methods described here are common to both air-hardening and hydraulic mortars, we focus above all on the latter, paying special attention to aspects relating to the chemical, mineralogical and petrographic investigation of the calcium silicate and aluminate hydrated phases that may indicate the use of one or other hydraulic binder in historic mortars, all this taking into account and discussing the practical aspects, drawbacks and limitations of each technique. European standards for the study of mortars are also addressed in this paper.