Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus
Copyright policy )Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus
We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats.
Recombination, Genetic, Restriction Mapping, Gene Products, gag, Herpesviridae Infections, Immunodeficiency Virus, Feline, Cat Diseases, Genes, gag, Polymerase Chain Reaction, Thymidine Kinase, Cell Line, Feline Acquired Immunodeficiency Syndrome, Cats, Animals, Varicellovirus, Promoter Regions, Genetic
Recombination, Genetic, Restriction Mapping, Gene Products, gag, Herpesviridae Infections, Immunodeficiency Virus, Feline, Cat Diseases, Genes, gag, Polymerase Chain Reaction, Thymidine Kinase, Cell Line, Feline Acquired Immunodeficiency Syndrome, Cats, Animals, Varicellovirus, Promoter Regions, Genetic
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).3 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!