Characterization of Trichomonas vaginalis virus proteins in the pathogenic protozoan T. vaginalis

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1998 English Publisher:Springer Science and Business Media LLCJournal:Archives of Virology, volume 143, pages 963-970 (issn: 0304-8608, eissn: 1432-8798,

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Authors: H W, Liu; Y D, Chu; J H, Tai;

doi: 10.1007/s007050050345

pmid: 9645201

Characterization of Trichomonas vaginalis virus proteins in the pathogenic protozoan T. vaginalis

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Abstract

The 4.6-kb double-stranded (ds) RNA of Trichomonas vaginalis virus (TVV)-T1 has been shown to encode two overlapping genes, cap and pol. In this study, a serum for specifically detecting viral cap gene product was raised against a recombinant protein, and sera for specifically detecting pol gene product were raised against synthetic oligopeptides. A 75-kDa major protein and a 160-kDa minor protein were detected by anti-CAP serum in a TVV-T1 sample, indicating that the 75-kDa protein is the viral capsid protein. The 160-kDa protein alone was also detected by two distinct anti-POL sera, indicating that the pol gene is expressed as a CAP-POL fusion protein. These results suggest that the TVV-T1 genome is arranged into a cap-pol organization in a manner similar to that of viruses in family Totiviridae.

Related Organizations

Development Center for Biotechnology
Taiwan
Academia Sinica
Taiwan

Keywords

Genes, Viral, Gene Products, pol, Genome, Viral, Antibodies, Viral, Recombinant Proteins, Viral Proteins, Capsid, Escherichia coli, Trichomonas vaginalis, Animals, Humans, RNA Viruses, Totiviridae

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