
pmid: 9694380
A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase K allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.
Electrophoresis, Agar Gel, Cetrimonium, Muscles, Povidone, DNA, Helminth, Polymerase Chain Reaction, Deoxyribonuclease EcoRI, Cetrimonium Compounds, Animals, Parasitology, Ascaris suum
Electrophoresis, Agar Gel, Cetrimonium, Muscles, Povidone, DNA, Helminth, Polymerase Chain Reaction, Deoxyribonuclease EcoRI, Cetrimonium Compounds, Animals, Parasitology, Ascaris suum
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