Comparison of the internal transcribed spacer, ITS 1, from Toxoplasma gondii isolates and Neospora caninum

descriptionPublicationkeyboard_double_arrow_right Article 05 Mar 1997 Netherlands, Belgium Publisher:Springer Science and Business Media LLCJournal:Parasitology Research, volume 83, pages 285-289 (issn: 0932-0113, eissn: 1432-1955,

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Authors: Homan, WL; Limper, L; Verlaan, M; Borst, A; Vercammen, M; van Knapen, F;

doi: 10.1007/s004360050248

pmid: 9089727

Comparison of the internal transcribed spacer, ITS 1, from Toxoplasma gondii isolates and Neospora caninum

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Abstract

The internal transcribed spacer (ITS1) region and the 5' part of the 5.8S ribosomal RNA gene of the ribosomal DNA repeat from 20 Toxoplasma gondil isolates was sequenced and found to be identical in all isolates, independent of host origin or virulence to mice. The ITS1 region from the closely related coccidian parasite Neospora caninum differed in 22% of its nucleotides. Hence, the ITS1 region provides a good marker for the distinction of T. gondii and N. caninum but is not useful for epidemiology studies of T. gondii.

Countries

Netherlands, Belgium

Related Organizations

National Institute for Public Health and the Environment
Netherlands
Vrije Universiteit Brussel
Belgium

Keywords

Base Sequence, toxoplasma gondii, Molecular Sequence Data, Neospora, DNA, Protozoan, DNA, Ribosomal, RNA, Ribosomal, 5.8S, Mice, Sequence Homology, Nucleic Acid, Animals, Humans, Toxoplasma

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