Asymmetrical labeling of giant phospholipid vesicles
Copyright policy )Asymmetrical labeling of giant phospholipid vesicles
The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles.
- University of Ljubljana Slovenia
4-Chloro-7-nitrobenzofurazan, Microscopy, Fluorescence, Phosphatidylcholines, Dithionite, Fluorometry, Membranes, Artificial, Phospholipids
4-Chloro-7-nitrobenzofurazan, Microscopy, Fluorescence, Phosphatidylcholines, Dithionite, Fluorometry, Membranes, Artificial, Phospholipids
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