Callus induction and somatic embryogenesis of Phalaenopsis

descriptionPublicationkeyboard_double_arrow_right Article 08 Apr 1998Publisher:Springer Science and Business Media LLCJournal:Plant Cell Reports, volume 17, pages 446-450 (issn: 0721-7714, eissn: 1432-203X,

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Authors: Y, Ishii; T, Takamura; M, Goi; M, Tanaka;

doi: 10.1007/s002990050423

pmid: 30736617

Callus induction and somatic embryogenesis of Phalaenopsis

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Abstract

Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer `Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g ⋅ l-1. Medium containing 200 ml ⋅ l-1 coconut water together with 40 g ⋅ l-1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.

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Kagawa University
Japan

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