Double-stranded RNA in the entomopathogenic fungus Metarhizium flavoviride
Copyright policy )Double-stranded RNA in the entomopathogenic fungus Metarhizium flavoviride
Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isogenic strains, with or without dsRNA, were submitted to virulence tests against the grasshopper Rhammatocerus schistocercoides. In contrast to what has been found in some phytopathogenic fungi, these dsRNA fragments did not cause hypovirulence to M. flavoviride.
Cell Nucleus, Polymorphism, Genetic, Genotype, Virulence, Single-Strand Specific DNA and RNA Endonucleases, RNA, Fungal, Grasshoppers, Ribonucleases, Ascomycota, Mutation, Animals, RNA, Double-Stranded
Cell Nucleus, Polymorphism, Genetic, Genotype, Virulence, Single-Strand Specific DNA and RNA Endonucleases, RNA, Fungal, Grasshoppers, Ribonucleases, Ascomycota, Mutation, Animals, RNA, Double-Stranded
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