Expression of MFA1 and STE6 is sufficient for mating type-independent secretion of yeast a-factor, but not mating competence
Copyright policy )Expression of MFA1 and STE6 is sufficient for mating type-independent secretion of yeast a-factor, but not mating competence
The yeast a-factor mating peptide and its transporter Ste6 are normally expressed only in MATa haploid cells. The a-factor is initially produced as a 36- or 38-residue peptide precursor and must undergo extensive post-translational processing to produce an active 12 amino-acid lipopeptide. To better understand the steps required for Ste6-dependent a-factor transport, we have reconstituted a-factor synthesis and transport in MATalpha haploids and MATa/alpha diploids. Ste6 and a-factor were stably expressed in MATalpha and MATa/alpha cells and the ectopically expressed a-factor was correctly processed. In addition, Ste6 was able to transport a-factor from all cell types, indicating that once expressed no other MATa-specific functions are required. However, despite significant levels of a-factor secretion, MATalpha cells are unable to support efficient mating.
- University of Iowa United States
Ploidies, Saccharomyces cerevisiae Proteins, Genotype, Genes, Fungal, Saccharomyces cerevisiae, Genes, Mating Type, Fungal, Pheromones, Fungal Proteins, Gene Expression Regulation, Fungal, Infertility, ATP-Binding Cassette Transporters, RNA, Messenger, Protein Precursors, Mating Factor, Peptides, Protein Processing, Post-Translational, Glycoproteins
Ploidies, Saccharomyces cerevisiae Proteins, Genotype, Genes, Fungal, Saccharomyces cerevisiae, Genes, Mating Type, Fungal, Pheromones, Fungal Proteins, Gene Expression Regulation, Fungal, Infertility, ATP-Binding Cassette Transporters, RNA, Messenger, Protein Precursors, Mating Factor, Peptides, Protein Processing, Post-Translational, Glycoproteins
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