Urea Hydrolysis and Suppressed Production of Thermostable Direct Hemolysin (TDH) by Vibrio parahaemolyticus Associated with Presence of TDH-Related Hemolysin Genes

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1997Publisher:Springer Science and Business Media LLCJournal:Current Microbiology, volume 34, pages 314-317 (issn: 0343-8651, eissn: 1432-0991,

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Authors: T, Okitsu; R, Osawa; S, Pornruangwong; S, Yamai;

doi: 10.1007/s002849900188

pmid: 9099633

Urea Hydrolysis and Suppressed Production of Thermostable Direct Hemolysin (TDH) by Vibrio parahaemolyticus Associated with Presence of TDH-Related Hemolysin Genes

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Abstract

A total of 18 strains of V. parahaemolyticus isolated from patients of past food poisoning cases occurring in Kanagawa Prefecture, Japan, were assayed for presence of the thermostable direct hemolysin (TDH) gene and the TDH-related hemolysin (TRH) genes (trh 1 and trh 2) with specific reference to their ability to hydrolyze urea and TDH production. A polymerase chain reaction assay revealed that all urea-hydrolyzing strains (9 strains) carried either trh 1 gene or trh 2 gene. The strains carrying the trh genes as well as the tdh gene produced TDH less by a factor of 4 to 16 than those carrying only the tdh gene, suggesting the expression of the tdh gene was suppressed by the presence of trh gene through a mechanism yet to be defined.

Related Organizations

Ministry of Public Health
Thailand
National Institute of Health of Thailand
Thailand

Keywords

Base Sequence, Genotype, Hydrolysis, Bacterial Toxins, Foodborne Diseases, Hemolysin Proteins, Phenotype, Bacterial Proteins, Genes, Bacterial, Humans, Urea, Vibrio parahaemolyticus, Latex Fixation Tests, DNA Primers

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