Phosphotransbutyrylase Expression in Bacillus megaterium
Copyright policy )Phosphotransbutyrylase Expression in Bacillus megaterium
The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponential growth phase. The branched amino acids isoleucine and valine activated Ptb expression. PtbBm was capable of using butyryl-CoA and 2-methyl-propionyl CoA as substrates. ActBm, a final sigma54 regulator from B. megaterium whose gene is situated upstream from the ptb gene, activated its expression.
- University of Buenos Aires Argentina
- Facultad de Ciencias Agrarias y Forestales Argentina
DNA-Binding Proteins, Kinetics, Phosphate Acetyltransferase, Bacillus megaterium, Sigma Factor, DNA-Directed RNA Polymerases, Gene Expression Regulation, Bacterial, RNA Polymerase Sigma 54, Amino Acids, Branched-Chain, Culture Media
DNA-Binding Proteins, Kinetics, Phosphate Acetyltransferase, Bacillus megaterium, Sigma Factor, DNA-Directed RNA Polymerases, Gene Expression Regulation, Bacterial, RNA Polymerase Sigma 54, Amino Acids, Branched-Chain, Culture Media
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