Plant-derived EpCAM antigen induces protective anti-cancer response
Copyright policy )Plant-derived EpCAM antigen induces protective anti-cancer response
Immunotherapy holds great promise for treatment of infectious and malignant diseases and might help to prevent the occurrence and recurrence of cancer. We produced a plant-derived tumor-associated colorectal cancer antigen EpCAM (pGA733) at high yields using two modern plant expression systems. The full antigenic domain of EpCAM was efficiently purified to confirm its antigenic and immunogenic properties as compared to those of the antigen expressed in the baculovirus system (bGA733). Recombinant plant-derived antigen induced a humoral immune response in BALB/c mice. Sera from those mice efficiently inhibited the growth of SW948 colorectal carcinoma cells xenografted in nude mice, as compared to the EpCAM-specific mAb CO17-1A. Our results support the feasibility of producing anti-cancer recombinant vaccines using plant expression systems.
- Thomas Jefferson University United States
- IE University Spain
Antibody Affinity, 610, Mice, Nude, Recombinant subunit vaccine, Cancer Vaccines, Antibodies, Mice, Antibody Specificity, Antigens, Neoplasm, Gene Expression Regulation, Plant, Cell Line, Tumor, Animals, Humans, Amino Acids, Cell Proliferation, Mice, Inbred BALB C, Immune Sera, and Proteins, Antibodies, Monoclonal, Epithelial Cell Adhesion Molecule, Colorectal cancer, Plant biotechnology, Immunotherapy, Beta vulgaris, Peptides, Colorectal Neoplasms, Cell Adhesion Molecules
Antibody Affinity, 610, Mice, Nude, Recombinant subunit vaccine, Cancer Vaccines, Antibodies, Mice, Antibody Specificity, Antigens, Neoplasm, Gene Expression Regulation, Plant, Cell Line, Tumor, Animals, Humans, Amino Acids, Cell Proliferation, Mice, Inbred BALB C, Immune Sera, and Proteins, Antibodies, Monoclonal, Epithelial Cell Adhesion Molecule, Colorectal cancer, Plant biotechnology, Immunotherapy, Beta vulgaris, Peptides, Colorectal Neoplasms, Cell Adhesion Molecules
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