Heterologous expression of metabotropic glutamate receptor subtype 1 in Saccharomyces cerevisiae
Copyright policy )Heterologous expression of metabotropic glutamate receptor subtype 1 in Saccharomyces cerevisiae
The upstream region of the isocitrate lyase gene (UPR-ICL) from the n-alkane-utilizing yeast Candida tropicalis serves as a useful promoter of gene expression in the yeast Saccharomyces cerevisiae. The production of rat metabotropic glutamate receptor 1alpha (mGluR1alpha), which belongs to the G-protein-coupled receptor (GPCR) family, was tested under the control of UPR-ICL. Expression of mGluR1alpha was found in recombinant clones and enhanced by replacing the signal sequence of mGluR1alpha with the corresponding region of the alpha-factor receptor (Ste2), which is a GPCR found in S. cerevisiae. Moreover, the membrane fraction from a recombinant clone associated with Vesl-1S/Homer-1a protein binds the mGluR1alpha in rat cerebellum. These results suggest that the UPR-ICL-controlled gene expression system is useful for heterologous GPCRs in S. cerevisiae.
- Mitsubishi Japan
- Kyoto University Japan
- Chubu University Japan
Recombinant Fusion Proteins, Cell Membrane, Saccharomyces cerevisiae, Protein Sorting Signals, Receptors, Metabotropic Glutamate, Isocitrate Lyase, Rats, Animals, Candida tropicalis, Cloning, Molecular, Promoter Regions, Genetic
Recombinant Fusion Proteins, Cell Membrane, Saccharomyces cerevisiae, Protein Sorting Signals, Receptors, Metabotropic Glutamate, Isocitrate Lyase, Rats, Animals, Candida tropicalis, Cloning, Molecular, Promoter Regions, Genetic
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