The complete primary structure of mouse 20S proteasomes
Copyright policy )The complete primary structure of mouse 20S proteasomes
The proteasome is a large multicatalytic proteinase that plays a role in the generation of peptides for presentation by major histocompatibility complex class I molecules. The 20S proteolytic core of mammalian proteasomes is assembled from a group of 17 protein subunits that generate a distinctive pattern of spots upon two-dimensional gel electrophoresis. The genes for most of these subunits have been cloned from humans and rats. We isolated cDNA clones for the mouse orthologues of ten of the subunits [PSMA1 (C2), PSMA2 (C3), PSMA3 (C8), PSMA4 (C9), PSMA5 (ZETA), PSMA6 (IOTA), PSMA7 (C6-I), PSMB2 (C7-I), PSMB3 (C10-II), and PSMB5 (X)] to complete the cloning of all of the mouse subunits. Using antisera raised against these subunits or their orthologues, we verified the identity of these proteins by two-dimensional NEPHGE-PAGE.
- University of Cincinnati United States
- Howard Hughes Medical Institute United States
- Virginia Commonwealth University United States
- University System of Ohio United States
Proteasome Endopeptidase Complex, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Protein Conformation, Molecular Sequence Data, Rats, Cysteine Endopeptidases, Mice, Species Specificity, Multienzyme Complexes, Animals, Humans, Electrophoresis, Gel, Two-Dimensional, Amino Acid Sequence, Cloning, Molecular, Phylogeny, DNA Primers
Proteasome Endopeptidase Complex, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Protein Conformation, Molecular Sequence Data, Rats, Cysteine Endopeptidases, Mice, Species Specificity, Multienzyme Complexes, Animals, Humans, Electrophoresis, Gel, Two-Dimensional, Amino Acid Sequence, Cloning, Molecular, Phylogeny, DNA Primers
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