Citrus paradisi peels were successively extracted with hexane, chloroform, acetone and methanol using a Soxhlet extractor for 8 h each. Thin layer chromatography (TLC) of hexane and chloroform extracts showed three spots with different concentrations; hence both the extracts were mixed and fractionated into alcohol soluble and insoluble fractions. Naringin was isolated from acetone and methanol extracts by column chromatography and its structure was confirmed by 1H and 13C NMR spectroscopic studies. Antibacterial activity of these fractions was evaluated against different bacteria. The alcohol soluble fraction was found to possess maximum activity followed by hexane extract. Extracts were more effective against Gram-positive than Gram-negative bacteria.