Fluoxetine-induced Ca 2+ signals in Madin-Darby canine kidney cells
Copyright policy )Fluoxetine-induced Ca 2+ signals in Madin-Darby canine kidney cells
The effect of fluoxetine on Ca2+ signaling in Madin-Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ probe. Fluoxetine increased [Ca2+]i concentration-dependently between 5 microM and 200 microM with an EC50 value of 40 microM. The response was reduced by external Ca2+ removal by 30%40%. In Ca2+-free medium pretreatment with 1 microM thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+ pump, abolished 100 microM fluoxetine-induced Ca2+ release. Addition of 3 mM Ca2+ to Ca2+-free medium increased [Ca2+]i when cells were pretreated with 100 microM fluoxetine. Suppression of 1,4,5-trisphosphate (IP3) formation by 2 microM U73122 (a phospholipase C inhibitor) did not affect 100 microM fluoxetine-induced Ca2+ release. Fluoxetine (5-100 microM) also increased [Ca2+]i in neutrophils, prostate cancer cells and bladder cancer cells from human and rat glioma cells.
- Pingtung Christian Hospital Taiwan
- National Yang Ming University Taiwan
- National Chiao Tung University Taiwan
- Kaohsiung Veterans General Hospital Taiwan
- National Sun Yat-sen University Taiwan
Time Factors, Neutrophils, Calcium-Transporting ATPases, Inositol 1,4,5-Trisphosphate, Endoplasmic Reticulum, Kidney, Pyrrolidinones, Cell Line, Adenosine Triphosphate, Dogs, Fluoxetine, Tumor Cells, Cultured, Animals, Humans, Thapsigargin, Calcium, Estrenes, Selective Serotonin Reuptake Inhibitors
Time Factors, Neutrophils, Calcium-Transporting ATPases, Inositol 1,4,5-Trisphosphate, Endoplasmic Reticulum, Kidney, Pyrrolidinones, Cell Line, Adenosine Triphosphate, Dogs, Fluoxetine, Tumor Cells, Cultured, Animals, Humans, Thapsigargin, Calcium, Estrenes, Selective Serotonin Reuptake Inhibitors
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