In the past few years, several methods have been reported for the gas chromatographic determination of acaricide residues in honey with different extraction procedure and detection system. The application of these methods to honey samples of different origins suggest that the treatment with the recommended dose during the adequate time, does not necessarily result in acaricide residues in honey. On the other hand, inadequate treatment of hives with incorrect amount and/or exposure time can result in pesticide contamination in honey. The objective of this work is to analyze the acaricide residues in honey samples in order to evaluate their level of contamination and so, to prevent toxicological risks that the treatment with the phystosanitary products might cause.