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https://doi.org/10.1007/bfb011...

Part of book or chapter of book . 2008 . Peer-reviewed

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Progress in Colloid & Polymer Science

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A computer program based on the psi function for model-independent analysis of sedimentation equilibrium distributions reflecting macromolecular interactions

Name: A computer program based on the psi function for model-independent analysis of sedimentation equilibrium distributions reflecting macromolecular interactions
Keywords: Sedimentation equilibrium, 1505 Colloid and Surface Chemistry, psi analysis, Heterogeneous association, 1606 Physical and Theoretical Chemistry, 2507 Polymers and Plastics, 2505 Materials Chemistry, Protein self-association, 1605 Organic Chemistry

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 02 Feb 2008 Australia Publisher:Steinkopff

Authors: Cölfen, H.; Winzor, D. J.;

doi: 10.1007/bfb0118013

A computer program based on the psi function for model-independent analysis of sedimentation equilibrium distributions reflecting macromolecular interactions

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Abstract

A computer program, written in BASIC, has been developed for the characterization of either solute self-association or the interaction between macromolecular acceptor and ligand. Simplicity and model independence of the procedure to evaluate the free concentration of smallest macromolecular species are features which are illustrated by analysis of simulated Rayleigh distributions based on α-chymotrypsin dimerization, and of simulated absorbance records of the distribution based on 1:1 interaction between trimethylamine dehydrogenase (acceptor) and electron transferring flavoprotein (ligand).

Country

Australia

Related Organizations

Max Planck Society
Germany
University of Queensland
Australia
University of Queensland
Australia
Max Planck Institute of Colloids and Interfaces
Germany
University of Queensland
Australia

Keywords

Sedimentation equilibrium, 1505 Colloid and Surface Chemistry, psi analysis, Heterogeneous association, 1606 Physical and Theoretical Chemistry, 2507 Polymers and Plastics, 2505 Materials Chemistry, Protein self-association, 1605 Organic Chemistry

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