Isolation of human CYP4F2 genomic DNA and its 5′ end regulatory region structure
Copyright policy )Isolation of human CYP4F2 genomic DNA and its 5′ end regulatory region structure
Human cytochrome P450 4F2 shows high regioselectivity in omega-hydroxylation of stearic acid and leukotriene B4. As a first step of its regulation study, human cytochrome P450 4F2 genomic DNA was isolated from liver of a person who was administered clofibrate for 10 years. From Southern hybridization, restriction enzyme digestion and sequencing experiments, isolated genomic DNA fragment was found to contain around 32 Kb DNA and more than 20 Kb of 5' end regulatory region. Sequences of the structural gene region revealed exon 1 and exon 2. Further regulation studies would elucidate the feedback mechanisms of the oxidative degradation of fatty acids, inflammatory response and the clearance of leukotriene B4 in the liver. Furthermore, regulation study of this gene could explain the species difference in response to peroxisome proliferator and help in the safety evaluation of peroxisome proliferating chemicals to human being.
- University of Suwon Korea (Republic of)
Base Sequence, Molecular Sequence Data, Chromosome Mapping, Nuclear Proteins, Receptors, Cytoplasmic and Nuclear, DNA, Regulatory Sequences, Nucleic Acid, Cytochrome P-450 Enzyme System, Humans, Amino Acid Sequence, Cytochrome P450 Family 4, Cloning, Molecular, DNA Probes, Transcription Factors
Base Sequence, Molecular Sequence Data, Chromosome Mapping, Nuclear Proteins, Receptors, Cytoplasmic and Nuclear, DNA, Regulatory Sequences, Nucleic Acid, Cytochrome P-450 Enzyme System, Humans, Amino Acid Sequence, Cytochrome P450 Family 4, Cloning, Molecular, DNA Probes, Transcription Factors
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).2 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!