AbstractScanning electron microscopy was used to study the crystal structure of hydrogenated Canola oil. Fixation of the samples was carried out at 10 C using osmium tetroxide vapor in a sealed container. Tristearin was used as a standard and was prepared in its three reported polymorphic forms α, β′ and β. Crystallization from ethyl acetate gave either a mixture of β′ and β or pure β′ polymorphic forms depending on the concentration of tristearin. Micrographs of these forms were obtained showing the morphology of the crystals. A Canola sample which was selectively hydrogenated to an iodine value of 70 was detergent fractionated, washed, dried and subjected to the same fixation procedure. Micrographs were obtained of its predominant β‐polymorphic form.