Tracking of phenol degrading genotype

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2001 English Publisher:Springer Science and Business Media LLCJournal:Environmental Science and Pollution Research, volume 8, pages 89-90 (issn: 0944-1344, eissn: 1614-7499,

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Authors: A, Kapley; H J, Purohit;

doi: 10.1007/bf02987299

pmid: 11400643

Tracking of phenol degrading genotype

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Abstract

A simple and rapid protocol has been developed for monitoring of the phenol degrading population in the environmental samples. PCR protocol uses the total DNA prepared from the samples as a template in the amplification reaction. Primers have been designed from the sequence data for Dmp and Phe operon encoding multi-component and single component phenol oxidizing system, respectively. The phenol degrading genotype in various samples have been demonstrated using the developed PCR protocol.

Related Organizations

National Environmental Engineering Research Institute
India

Keywords

DNA, Bacterial, Phenols, Pseudomonas, Operon, Environmental Pollutants, Templates, Genetic, Polymerase Chain Reaction, Sensitivity and Specificity, Environmental Monitoring

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