Apoptosis-inducing Costunolide and a novel acyclic monoterpene from the stem bark ofMagnolia sieboldii
Copyright policy )Apoptosis-inducing Costunolide and a novel acyclic monoterpene from the stem bark ofMagnolia sieboldii
In a course of obtaining more amount of bioactive costunolide and successive phytochemical isolation from Magnolia sieboldii (Magnoliaceae), a novel acyclic monoterpene 1 named deoxygeraniol [2,6(E)-dimethyl-2,6-octadiene] was isolated along with beta-sitosterol 3-O-linoleate (2), trilinolein (3) and high amount of costunolide (4) in the pure state. The structure of compound 1 was determined on the basis of spectroscopic data. Costunolide was found to induce apoptotic cell death in a dose-dependent manner by nucleosomal DNA ladder and flow cytometric analysis. Immunoblot analysis showed that the level of the anti-apoptotic protein, Bcl-2, was decreased, whereas the cleavage of poly-(ADP-ribose) polymerase was activated. Furthermore, the N-acetyl-L-cysteine antioxidant effectively prevented costunolide-induced cytotoxicity. These results suggest that costunolide-induced cell death is mediated by reactive oxygen species
- Kim Il-sung University Korea, Democratic People's Republic of
- Seoul National University Korea (Republic of)
- Eulji University Korea (Republic of)
- Kyung Hee University Korea (Republic of)
- Kanazawa University Japan
Cell Survival, Terpenes, Hydrolysis, Blotting, Western, Fatty Acids, Apoptosis, HL-60 Cells, DNA Fragmentation, Magnoliaceae, Acetylcysteine, Plant Epidermis, Mice, Caspases, Animals, Humans, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors, Poly(ADP-ribose) Polymerases, Reactive Oxygen Species, Sesquiterpenes
Cell Survival, Terpenes, Hydrolysis, Blotting, Western, Fatty Acids, Apoptosis, HL-60 Cells, DNA Fragmentation, Magnoliaceae, Acetylcysteine, Plant Epidermis, Mice, Caspases, Animals, Humans, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors, Poly(ADP-ribose) Polymerases, Reactive Oxygen Species, Sesquiterpenes
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