
doi: 10.1007/bf02931653
pmid: 15114873
The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23%) out of 77 crystalliferous strains of Bacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 degrees C with shaking in a medium of pH 6.5 with 4% colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase of B. thuringiensis.
Chitosan, Glycoside Hydrolases, Bacillus thuringiensis, Temperature, Chitin, Hydrogen-Ion Concentration, Molecular Weight, Enzyme Stability, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors, Pest Control, Biological
Chitosan, Glycoside Hydrolases, Bacillus thuringiensis, Temperature, Chitin, Hydrogen-Ion Concentration, Molecular Weight, Enzyme Stability, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors, Pest Control, Biological
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