descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2004 English Publisher:Springer Science and Business Media LLCJournal:Folia Microbiologica, volume 49, pages 191-193 (issn: 0015-5632, eissn: 1874-9356,

Authors: M, Piknová; P, Pristas; P, Javorský;

doi: 10.1007/bf02931400

pmid: 15227796

GATC-specific restriction-modification systems in ruminal bacteria

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Abstract

The GATC-specific restriction and modification activities were analyzed in 11 major bacterial representatives of ruminal microflora. Modification phenotype was observed in 13 out of 40 ruminal strains. MboI isoschizomeric restriction endonucleases were detected in 10 bacterial strains tested; three strains lacked any detectable corresponding endonuclease activity. The only examined strain of Mitsuokella multi-acida was found to possess a different type of endonuclease activity. This is the first report on restriction activity in ruminal treponemes M. multiacida and Megasphaera elsdenii.

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Keywords

Electrophoresis, Agar Gel, Rumen, Bacteria, Megasphaera, DNA, Viral, Animals, DNA Restriction-Modification Enzymes, Veillonellaceae, Deoxyribonucleases, Type II Site-Specific, Substrate Specificity

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