A simple procedure to obtain yeast hexokinase free of glucosephosphate isomerase and mannosephosphate isomerase
Copyright policy )A simple procedure to obtain yeast hexokinase free of glucosephosphate isomerase and mannosephosphate isomerase
A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. The fraction was used without further purification in the determination of glucose, either free or in a mixture with fructose and mannose. The results were similar to those obtained with pure commercial hexokinase.
Mannose-6-Phosphate Isomerase, Ammonium Sulfate, Hexokinase, Mutation, Glucose-6-Phosphate Isomerase, Chemical Precipitation, Saccharomyces cerevisiae
Mannose-6-Phosphate Isomerase, Ammonium Sulfate, Hexokinase, Mutation, Glucose-6-Phosphate Isomerase, Chemical Precipitation, Saccharomyces cerevisiae
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).0 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!