A magnetizable solid phase for enzyme extraction
Copyright policy )A magnetizable solid phase for enzyme extraction
A method for the convenient and reliable preparation of magnetizable agarose beads containing iron particles is described. The beads were treated with the triazine dye, Reactive Red 120, and the matrix was examined for the ability to extract proteins from crude preparations using lactate dehydrogenase from porcine muscle as a model. The recovery and specific activity values of enzyme obtained using this matrix and magnetic field separation were significantly greater than those for enzyme purified by centrifugation and conventional dye ligand chromatography.
- Queen's University Belfast United Kingdom
L-Lactate Dehydrogenase, Swine, Triazines, Iron, Muscles, Sepharose, Enzymes, Magnetics, Evaluation Studies as Topic, Animals, Adsorption, Coloring Agents, Biotechnology
L-Lactate Dehydrogenase, Swine, Triazines, Iron, Muscles, Sepharose, Enzymes, Magnetics, Evaluation Studies as Topic, Animals, Adsorption, Coloring Agents, Biotechnology
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).12 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!